evorion CellCity

Functional Phenomics

to decipher

cell-cell interactions

Our CellCity System allows you to array and track thousands of cells as they interact with each other and respond to their shared surrounding environment.

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a cellular universe within
thousands of gel beads

Chip in Hand

Cellular communication matters in vivo.
So why shouldn’t it in vitro?

Quantifying biological parameters in a population of cells obscures critical insights into how neighboring cells in a microenvironment influence function, particularly in heterogeneous samples. You can reveal these obscured interactions by performing single-cell measurements on a massively parallel platform.

CellCity is a unique microfluidics concept, which was developed for the analysis of live cells. It documents the actions of thousands of cells and cell pairs in a defined arrangement and environment at single-cell resolution. It allows you to control cell proximity in order to elucidate cell-cell interactions and track phenotype changes over time and space. Explore new frontiers in cell-based biomedical research. That is the transformative power of CellCity.

  • CELL INTERACTION ANALYSIS

    Co-position cells to study hundreds of interactions simultaneously.

     

  • FUNCTIONAL PHENOMICS

    Link changing functional phenotypes to underlying genotypes.

     

  • IN-DEPTH POPULATION STUDIES

    Reveal how cells function in a physiologically accurate context.

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About Functional Phenomics

This unique workflow links a single cell‘s functional phenotype with multi-omics data thereby developing knowledge for new breakthroughs in cell therapy development.

Current single-cell multi-omics technologies fail to directly link a single cell’s multi-omics data to changes in its functional phenotype over time. This is where our innovative workflow excels. We enable to simultaneously analyze multiple phenotypes (cellular functions, intracellular protein expression, surface and secreted protein profiles), the cellular interactions of thousands of cells in highly parallelized manner and to link those insights directly to each cell’s underlying genetic and transcriptional makeup.

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One Platform. Endless
Applications.

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Cell-Cell Interactions

 

Study cellular communication at single-cell resolution, e.g. during EMT in cancer metastasis.

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Cell Killing

 

Discover and explore serial killing events of individual CAR-T cells.

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Cell Division

 

Use cell division as a functional readout to reveal the role of cell proliferation in disease.

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PHAGOCYTOSIS

 

Visualize at single-cell resolution how, e.g. a macrophage phagocytoses a cancer cell.

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WorkFlow

Simple workflow. Easy setup. Powerful research tool.

Workflow Image 1 Cell Encapsulation
Step 1

 

Cell encapsulation:

Encapsulate individual, pairs, or groups of cells in a gel bead which acts as a protective and diffusible microenvironment. Work with a range of cell sizes, from prokaryotes to eukaryotes.

Workflow Image 2 Gel Bead Trapping
Step 2

 

Gel bead trapping:

Immobilize up to 2000 cell-laden gel beads in microfluidic traps on the CellCity Array Chip in order to form an array in which each gel bead is uniquely identified by its precisely defined position.

Workflow Image 3 Cell Culture
Step 3

 

Cell CULTURE:

Culture cells under tightly controlled conditions with the optional ability to stimulate cells. Monitor cell behaviour and function using time-lapse microscopy.

Workflow Image 4 Cell Analysis
Step 4

 

Cell ANALYSIS:

Couple endpoint measurements with time-lapse data, while simultaneously capturing the responses of single cells in multiparametic analysis, such as time-lapse fluorescent imaging and immunohistochemistry.

Workflow Image 5 Cell Retrieval
Step 5

 

CELL RETRIEVAL:

Easily retrieve the gel beads in which the cells are contained for subsequent downstream analysis or cell expansion.

The workflow of CellCity is akin to the busy streets of any big city around the world, where we all meet, interact, observe, and change with the influence of the people we meet, the experiences we share, and the impacts we have on each other.

If you want to build your own cell city,
Take a look at the custom workflow services we provide

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thesetup

The Software – The Brain – The Incubator – The Array Chip

Precision engineering makes CellCity a powerful analysis tool enabling functional phenomics, but its smart design is what makes it a seamless experience for the user. With its plug-and-play format, carefully planned components, and easy portability, the entire system can easily plug right into any inverted imaging system, lab setup, or study routine.

CELLCITY SOFTWARE SOLUTIONS

User-friendly, proprietary PC-software communicates with the CellCity Control Unit to regulate and perform all essential workflow steps. Also, it helps researchers to minimize experimental steps and accelerate analyses in real-time.

  • Built-in performance-checks ensure reliable and accurate assay outcomes
  • Accelerates and simplifies workflows e.g. with cell counting and gel bead analysis tools
  • Artificial intelligence (AI)-based position selection tool: CellCity Scout-AI, automatically select hundreds of positions of interest on the CellCity Array Chip for time-lapse imaging

THE INCUBATOR
ON YOUR INVERTED IMAGING SYSTEM

The CellCity Incubator holds the CellCity Array Chip and maintains the relevant experimental conditions for long-term, live-cell analysis.

  • A complete, miniature incubator (37°C, 5% CO₂). No need for any other chamber.
  • Designed in a microtiter plate format to fit on your inverted microscope setup.
  • Controlled and optimized heating to work with low volumes and temperature sensitive samples
  • The CellCity Incubator can be easily moved to a cell culture hood for uninterrupted sterile conditions and handling

THE CHIP

The CellCity Array Chip is a high-precision microfluidic device that places single, pairs and groups of cells into an array of gel beads for sterile culture and analysis. No microfluidics experience required

  • Cell-laden gel beads are captured in the 425 microfluidic double-traps of 4 microfluidic channels to analyze up to 3400 gel beads at a time
  • Accommodates different cell types and cell lines, in particular primary cells (e.g. HUVEC, breast cancer cells, brain tumors, CAR-T cells, macrophages, leukemia cells and even bacteria)
  • Includes a high-resolution temperature sensor integrated at the cell culture site exactly where the correct temperature is needed
  • Augments insights with endpoint data by making it easy to stain cells (e.g., immunostaining, surface staining) after time-lapse (fluorescent) imaging

THE BRAIN
CONTROL UNIT

The CellCity Control Unit automates all workflow steps essential for reliable cell culture and analysis and executes the experimental design through integrated control software.

  • Powerful and fast, traps thousands of cell-laden gel beads on a CellCity Array Chip within a few minutes
  • Light-weight, easily portable and designed for a quick plug-and-play setup
  • Brings microfluidic cell culture to any lab (intuitive user experience, automated flow control and priming, bubble-free operation)
  • Precision engineering delivers uniform perfusion to ensure homogeneous culture conditions
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your advantages

More insights from every cell enabled by functional phenomics

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CO-POSITION TWO CELLS

Easily enclose two cells in one bead to monitor communication & regulation

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PRESERVE THE 3D SPACE AROUND CELLS

Easily control & maintain their chemical & cellular microenvironment

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CONTROL YOUR EXPERIMENT

Quickly set, adjust, and change conditions to examine how cells respond

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LINK A CELL’S GENES & FUNCTION

Track reporter genes and other modifications of single cells upon response to stimuli

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USE YOUR EXISTING PROTOCOLS

No need to start from scratch. CellCity works with your current bioassays & imaging setup

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GAIN COMPLEX INSIGHTS FAST

1 chip, 4 channels, 2000 beads. Generate large, meaningful datasets in each run

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SEE PHENOTYPIC HETEROGENEITY IN POPULATIONS

Show phenotypic variation across time & space, and even track monoclonal cells

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YOUR IDEAS. YOUR CELLS. YOUR ASSAY

Answer questions with rare, eukaryotic, prokaryotic, primary, or other cells

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Conduct reliable live-cell studies

Gentle processing, uniform perfusion, and stable cultures ensure cell integrity & vitality

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LOW CELL & SAMPLE INPUT

Impactful insights from as little as a 10 μl sample volume

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NOT ANOTHER EXPENSIVE DEVICE

Boost the capabilities of your current microscope or confocal setup instead

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USE REAGENTS EFFICIENTLY

Targeted and uniform perfusion culture saves reagents
(200 μl per condition per day)

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REDUCE TIME-INTENSIVE WORK

A simple, robust, & partly hands-free workflow saves time and avoids reworks

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BOOST YOUR VISIBILITY

Learn more from every study. Produce data faster. Publish insights sooner

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LESS INPUT, LESS WASTE

Miniaturized assays result in powerful outcomes with huge power savings

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COMPLETELY STERILE CELL HANDLING

The self-contained chip makes it easy to prevent contamination, even in shared labs

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FLEXIBLE AND PORTABLE

Barely bigger than a book, CellCity is portable & easy to plug in wherever you need it

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SIMPLE WORKFLOW FOR ANY USER

Designed for use in busy labs, anyone can prepare and run experiments with evorion

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ETHICALLY ALIGNED

Fewer cells required for experiments, making applying the 3Rs easier

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